Titration for Vitamin C
I make this content to teach you how to do a titration to analyze the vitamin C content of L-ascorbic acid, which is a vitamin C solution.
Please use the personal protection equipment. Provided as directed by your teacher.
- 2 100Ml volumetric Flasks
- Distilled water
- A 100ml measuring cylinder
- A 400ml beaker
- 2 100ml beakers
- A 100ml conical flask
- Dropping pads
- A spatula
- A marker pen
- A 10 mL syringe
- A funnel
- 1% L-ascorbic acid
- Dichlorophenol – Indophenol (DC PIP)
Making the standard Solutions
Way out, Ig of L-ascorbic acid and 0.1g of DC pip. Add 100ml of distilled water to the 1g of L-ascorbic acid.
Pour the solution into a volumetric flask and make up to the mark. When filling the volumetric flask ensure the bottom of the meniscus is level with the graduation. Convert the volumetric flask to ensure no un-dissolved solid remains.
A small amount of water was added to the DC Pip in a 100ml beaker to ensure the dissolving process begins. Stirl the solution into a 100ml volumetric flask and make up to the mark.
Conditioning the Equipment
Put a small amount of vitamin C solution in the burette then invert burette. Dispose the unwanted solution in a sink.
Put 25mL of DC pip into 100ml conical flask.
Performing the Titration
Fill the burette with vitamin C solution to a point above the zero graduation. Well, access Solutions flow through the burette.
At the bottom of the meniscus to set on level with the zero graduation allowing excess solution to flow through the burette ensures that the burette is conditioned beyond the tap, discard the excess solution as this may be contaminated from previous use.
Results from the titration can be presented in the table as follows.